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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in lots of tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was identified to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a significant purpose in selling mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are explained. Two additional splice variants were described but thorough nucleotide sequence information and facts is not really nonetheless out there.
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Blocking peptides are peptides that bind especially towards the focus on antibody and block antibody binding. These peptide usually has the epitope acknowledged because of the antibody.
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This tutorial outlines more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...
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This guidebook outlines more doable optimization of mobile and immunoassay parameters to be sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind to the epitope around the focus on protein. By comparing the staining within the blocked antibody vs . the antibody by itself, one can see which staining is unique.
Target Information Cyclin E belongs to the extremely conserved cyclin family members, whose members are characterised by a dramatic periodicity in protein abundance from the read more cell cycle. Cyclins function as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation patterns which contribute into the temporal coordination of every mitotic event. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary which is degraded as cells progress by way of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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